An inexpensive apparatus for growing photosynthetic microorganisms in exotic atmospheres

Given the need for a light source, cyanobacteria and other photosynthetic microorganisms can be difficult and expensive to grow in large quantities. Lighted growth chambers and incubators typically cost 50-100% more than standard microbiological incubators. Self-shading of cells in liquid cultures prevents the growth of dense suspensions. Growing liquid cultures on a shaker table or lighted shaker incubator achieves greater cell densities, but adds considerably to the cost. For experiments in which gases other than air are required, the cost for conventional incubators increases even more. We describe an apparatus for growing photosynthetic organisms in exotic atmospheres that can be built relatively inexpensively (approximately 100 dollars U.S.) using parts available from typical hardware or department stores (e.g., Wal-mart or K-mart). The apparatus uses microfiltered air (or other gases) to aerate, agitate, and mix liquid cultures, thus achieving very high cell densities (A750 > 3). Because gases are delivered to individual culture tubes, a variety of gas mixes can be used without the need for enclosed chambers. The apparatus works with liquid cultures of unicellular and filamentous species, and also works with agar slants.     

Biogeochemistry of hypersaline springs supporting a mid-continent marine ecosystem: an analogue for martian springs?

Hypersaline springs that host unique mid-continent marine ecosystems were examined in central Manitoba, Canada. The springs originate from a reflux of glacial meltwater that intrudes into underlying bedrock and dissolved buried salt beds. Two spring types were distinguished based both on flow rate and geochemistry. High flow springs (greater than 10 L/s) hosted extensive marine microbial mats, which were dominated by algae but also included diverse microbes. These varied somewhat between springs as indicated by changes in profiles of fatty acid methyl esters. Culture studies confirmed the presence of sulfate-reducing bacteria in sediments at the high flow sites. In contrast, low flow springs were affected by solar evaporation, increasing salinity, and temperature. These low flow springs behaved more like closed nutrient-limited systems and did not support microbial mats. Direct comparison of the high and low flow springs revealed interesting implications for the potential to record biosignatures in the rock record. High flow springs have abundant, well-developed microbial mats, which desiccate and are cemented along the edges of the spring pools; however, the high mass flux overwhelms any geochemical signature of microbial activity. In contrast, the nutrient-limited low flow sites develop strong geochemical signatures of sulfate reduction, even in the absence of microbial mats, due to less dilution with the lower flows. Geochemical and physical evidence for life did not correlate with the abundance of microbial life but, rather, with the extent to which the biological system formed a closed ecosystem.     

Compatibility of Terrestrial Reference Frames used in GNSS broadcast messages during an 8 week period of 2019

The operational Terrestrial Reference Frames (TRFs) realized through the evaluation of broadcast ephemerides for GPS, GLONASS, Galileo, BeiDou-2 and BeiDou-3 have been compared to IGS14, the TRF realized by the International GNSS Service (IGS). The TRFs realized by the GPS, GLONASS, Galileo, and BeiDou-2 and BeiDou-3 broadcast ephemerides are the orbital realizations of WGS 84 (G1762′), PZ90.11, GTRF19v01, and BDCS respectively. These TRFs are compared using up to 56 days of data (21 July-14 Sept 2019) at a 5 or 15-min rate. The operational TRFs are compared to IGS14 in a 7-parameter similarity (Helmert) transformation. Numerical results show that the operational GNSS TRFs differ from IGS14 at a level no greater than 4 cm for Galileo, 6 cm for GPS and BeiDou-3, 13 cm for GLONASS, and 48 cm for a limited set of BeiDou-2 Medium Earth Orbit (MEO) vehicles.     

Origin of Molecular Oxygen in Comets: Current Knowledge and Perspectives

The Rosetta Orbiter Spectrometer for Ion and Neutral Analysis (ROSINA) instrument onboard the Rosetta spacecraft has measured molecular oxygen (O₂) in the coma of comet 67P/Churyumov-Gerasimenko (67P/C-G) in surprisingly high abundances. These measurements mark the first unequivocal detection of O₂ in a cometary environment. The large relative abundance of O₂ in 67P/C-G despite its high reactivity and low interstellar abundance poses a puzzle for its origin in comet 67P/C-G, and potentially other comets. Since its detection, there have been a number of hypotheses put forward to explain the production and origin of O₂ in the comet. These hypotheses cover a wide range of possibilities from various in situ production mechanisms to protosolar nebula and primordial origins. Here, we review the O₂ formation mechanisms from the literature, and provide a comprehensive summary of the current state of knowledge of the sources and origin of cometary O₂.     

Following the kinetics: iron-oxidizing microbial mats in cold icelandic volcanic habitats and their rock-associated carbonaceous signature

Icelandic streams with mean annual temperatures of less than 5 °C, which receive the cationic products of basaltic rock weathering, were found to host mats of iron-cycling microorganisms. We investigated two representative sites. Iron-oxidizing Gallionella and iron-reducing Geobacter species were present. The mats host a high bacterial diversity as determined by culture-independent methods. β-Proteobacteria, Actinobacteria, α-Proteobacteria, and Bacteroidetes were abundant microbial taxa. The mat contained a high number of phototroph sequences. The carbon compounds in the mat displayed broad G and D bands with Raman spectroscopy. This signature becomes incorporated into the weathered oxidized surface layer of the basaltic rocks and was observed on rocks that no longer host mats. The presence of iron-oxidizing taxa in the stream microbial mats, and the lack of them in previously studied volcanic rocks in Iceland that have intermittently been exposed to surface water flows, can be explained by the kinetic limitations to the extraction of reduced iron from rocks. This type of ecosystem illustrates key factors that control the distribution of chemolithotrophs in cold volcanic environments. The data show that one promising sample type for which the hypothesis of the existence of past life on Mars can be tested is the surface of volcanic rocks that, previously, were situated within channels carved by flowing water. Our results also show that the carbonaceous signatures of life, if life had occurred, could be found in or on these rocks.     

Remote sounding of atmospheric gravity waves with satellite limb and nadir techniques

Recent advances in satellite techniques hold great potential for mapping global gravity wave (GW) processes at various altitudes. Poor understanding of small-scale GWs has been a major limitation to numerical climate and weather models for making reliable forecasts. Observations of short-scale features have important implication for validating and improving future high-resolution numerical models. This paper summarizes recent GW observations and sensitivities from several satellite instruments, including MLS, AMSU-A, AIRS, GPS, and CLAES. It is shown in an example that mountain waves with horizontal wavelengths as short as 30 km now can be observed by AIRS, reflecting the superior horizontal resolution in these modern satellite instruments. Our studies show that MLS, AMSU-A and AIRS observations reveal similar GW characteristics, with the observed variances correlated well with background winds. As a complementary technique, limb sounding instruments like CRISTA, CLAES, and GPS can detect GWs with better vertical but poorer horizontal resolutions. To resolve different parts of the broad GW spectrum, both satellite limb and nadir observing techniques are needed, and a better understanding of GW complexities requires joint analyses of these data and dedicated high-resolution model simulations.     

The position sensitive low energy detector on board the ZEBRA telescope

In order to improve the low energy capability (15 ÷ 150 KeV) of the balloon borne “ZEBRA” low energy gamma imaging telescope (150 KeV-20 MeV), a large area, high spectral resolution (5% at 60 KeV), low background detector has been designed and is now under development.It consists of two MultiWire Spectroscopic Proportional Counter (SPC), escape gated, that have a sensitive area of 6000 cm², and are placed above the large area array of sodiumiodide position sensitive elements.     

Mature systems for small satellite missions

Over the past several years Satellites International has developed an integrated suite of satellite sub-systems and small satellite buses. The sub-systems include S-band communications, attitude sensing and control, power conversion and distribution, and on-board data handling. They are inherently modular and readily adaptable to different satellite configurations, a concept known as semi-standardisation. This concept has been adopted by two generic low-cost buses: MicroSIL for satellites in the mass range 40–80kg; and MiniSIL for satellites in the range 100–500kg. Their architecture is based on the semi-standard sub-systems, but easily modified to utilise sub-systems from other manufacturers. They can support all stabilisation methods including spinning, 3-axis control and gravity gradient and are adaptable to a wide variety of missions including Earth resources, scientific, communications and technology demonstration. The Company also manufactures a range of low cost ground support equipment and complete ground stations to complement the space-borne systems.